rabbit anti socs1  (Proteintech)

Journal: Parasites & Vectors

Article Title: Leptin enhances the efficacy of glucantime to modulate macrophage polarization toward the M1 phenotype in Leishmania tropica -infected macrophages

doi: 10.1186/s13071-025-07004-6

Figure Lengend Snippet: The SOCS expression by Leishmania -infected THP1 cells treated with leptin, glucantime, and their combination. A mRNA expression of SOCS3. B Protein expression of SOCS3. C mRNA expression of SOCS1. D protein expression of SOCS1. To assess mRNA and protein expression of SOCS molecules, Leishmania tropica -infected macrophages were treated with leptin, glucantime, or their combinations and incubated at 37 °C for 24 or 72 h. The total RNA was extracted at 24 h and then converted to cDNA. Real-time PCR was used to determine the mRNA expression of SOCS1 and SOCS3. To determine SOCS1 and SOCS3 production, the cell supernatants were harvested at 72 h, and the amounts of SOCS molecules were assessed using the ELISA method. The experiments were performed in triplicate, and the results are expressed as mean ± SD. One-way ANOVA followed by Student's t-test was used to compare variables between cultures (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: For immunofluorescence labeling, cells were incubated overnight at 4 °C with the following primary antibodies: a rabbit polyclonal antibody against SOCS1 (Elabscience, E-AB-32936, USA) and a mouse monoclonal antibody against SOCS3 (Santa Cruz Biotechnology, 6A463, USA).

Techniques: Expressing, Infection, Incubation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Parasites & Vectors

Article Title: Leptin enhances the efficacy of glucantime to modulate macrophage polarization toward the M1 phenotype in Leishmania tropica -infected macrophages

doi: 10.1186/s13071-025-07004-6

Figure Lengend Snippet: Immunofluorescence staining patterns of SOCS1 and SOCS3 expression in Leishmania -infected THP1 cells treated with leptin, glucantime, and their combination. A DAPI nuclear staining (blue), immunofluorescence green and red colors indicate SOCS1 and SOCS3 expression, respectively. Immunofluorescence staining illustrating the impact of leptin, glucantime, and their combination treatments on SOCS1 (FITC, green) and SOCS3 (CY3, red) protein expression in Leishmania tropica -infected macrophages after 72 h. Fluorescence intensity was analyzed via ImageJ, with results expressed as mean ± SD and statistically compared using one-way ANOVA and Student’s t-test (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: For immunofluorescence labeling, cells were incubated overnight at 4 °C with the following primary antibodies: a rabbit polyclonal antibody against SOCS1 (Elabscience, E-AB-32936, USA) and a mouse monoclonal antibody against SOCS3 (Santa Cruz Biotechnology, 6A463, USA).

Techniques: Immunofluorescence, Staining, Expressing, Infection, Fluorescence

Journal: Frontiers in Oncology

Article Title: Ferroptosis-related gene signature-based subtype identification of triple-negative breast cancer to prioritize treatment strategies

doi: 10.3389/fonc.2025.1541119

Figure Lengend Snippet: SOCS1 and NOX4 were correlated with the TME reshape in TNBC. (A–E) Pearson’s correlation analysis of the mRNA expression of SOCS1 and immune checkpoints (PD-1, PD-L1, CTLA-4, LAG3, and TIM3). (F–H) Pearson’s correlation analysis of the mRNA expression of NOX4 and immune checkpoints (PD-1, IDO1, and LAG3). (I) IHC detected the expression of SOCS1, NOX4, PD-1, and CD3 in two immune phenotypes of TNBC according to the spatial distribution of CD8 + T cells. Representative co-stained images are shown, and the scale bar corresponds to 500 μm. (J) The expression of SOCS1, NOX4, PD-1, and CD3 in two phenotypes of TNBC detected by four-color IF. Representative co-stained images are displayed, and the scale bar corresponds to 20 μm. (K) The expression levels of the SOCS1 gene across 11 TNBC cell lines using the DepMap portal ( https://depmap.org/portal ). (L) The knockdown efficiency of SOCS1 in MDA-MB-231 cells. (M) Cell viability in MDA-MB-231 cells (either control or shSOCS1) treated with RSL3 was assessed using the CCK-8 assay. **p < 0.01. TME, tumor microenvironment; TNBC, triple-negative breast cancer; IHC, immunohistochemical; IF, immunofluorescence.

Article Snippet: The knockdown efficiency was evaluated by Western blotting analysis using an antibody against human SOCS1 (Cat# 55313, Cell Signaling Technology, Inc., Danvers, MA, USA).

Techniques: Expressing, Staining, Knockdown, Control, CCK-8 Assay, Immunohistochemical staining, Immunofluorescence